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JK-1
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Cat.No.
CSC-C0403
Description
Established from the biopsy material of the shoulder tumor of a 62-year-old man with chronic myeloid leukemia (CML) in erythroid blast crisis in 1987; described to produce hemoglobin; cells are described to carry t(9;22) leadin to BCR-ABL1 e13-a2 (b2-a2) fusion gene
Species
Human
Source
chronic myeloid leukemia in blast crisis
Culture Properties
suspension
Morphology
round single cells growing in suspension (cell pellet shows reddish color hemoglobin)
Karyotype
Human hyperdiploid karyotype without significant polyploidy - 48(45-48)<2n>XY, +8, +22, t(1;7)(q10;q10), der(9)t(9;22)(q34;q11), der(22)t(9;22)(q34;q11)x2 - carries two Ph - resembles published karyotype
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD13 (+), CD14 -, CD15 -, CD19 -, CD33 +, CD34 +, CD41 (+), CD42 (+), CD71 +, CD235a +
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV
Immunology: CD3 -, CD13 (+), CD14 -, CD15 -, CD19 -, CD33 +, CD34 +, CD41 (+), CD42 (+), CD71 +, CD235a +
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
- Q & A
- Customer Review
Q:
How does immunohistochemistry detect tumor markers?
A:
IHC reveals more than a standard biopsy test. It enables pathologists to determine the exact type and subtype of cancer by looking for unique markers within cancer cells. Markers are identified using antibodies, a type of protein that locates and binds with antigens (which are the markers found on cells).
Average Rating: 5.0 | 1 Scientist has reviewed this product
Easy to culture
Following the instructions, these cells are easy to culture.
04 Aug 2022
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