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HDLM-2

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Cat.No.
CSC-C0201
Description
Established from the pleural effusion of a 74-year-old man with Hodgkin lymphoma (nodular sclerosing, stage IV) in 1982
Source
Hodgkin lymphoma
Recommended Medium
Culture Properties
suspension
Morphology
single, round to polygonal cells in suspension; heterogeneity in cell size, 15-100 μm or larger; many bi- and polynucleated cells
Images
HDLM-2 STR DNA Profile
HDLM-2 STR DNA Profile
STR DNA Profile
Amelogenin X,Y
CSF1PO 11,12
D5S818 11
D7S820 8,10
D13S317 11,12
D16S539 12
TH01 6,7
TPOX 8
vWA 19
Karyotype
Remarkable human hypodiploid cell line with large number of rearrangements involving all chromosomes except ch 10 - 36(35-38)<2n>X, der(Y), breakpoints at: 1p21, 1q11, 2p14, 2q37, 3q21, 3p14, 3q11, 3q25.2, 3q27, 4q25, 5q11, 5q35, 6q15/16, 7p14, 8q21.1, 8q
Markers
CD2 +, CD3 -, CD4 (+), CD13 -, CD14 -, CD15 +, CD19 -, CD21 -, CD25 +, CD30 +, HLA-DR +
Sequence Variations
TP53 p.Ser261fs c.?(782delins)
Applications
a. Hodgkin lymphoma related reserach
b. Cell-Based assay
c. In vivo efficacy study
Quality Control
Mycoplasma: Fluorescence (DAPI) test: negative; Mycoplasma specific PCR: negative; Selective biochemical test: negative
Viruses: ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Storage and Shipping
Ship in dry ice.
Store in liquid nitrogen.
BioSafety Level
BSL–1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.,
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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