Established in 1991 from the pleural effusion (rich in Hodgkin/Reed-Sternberg cells) from a 29-year-old patient with nodular sclerosing Hodgkin lymphoma (stage IIIb) refractory to therapy
Human highly rearranged hyperdiploid karyotype with 24% polyploidy - 52-55<2n>X/Xdel(X)(p11), +1, +2, +5, +6, +8, +9, -17, -17, -18, +19, +20, +21, +22, +2mar, der(1)t(1;3)(q32;p21), der(2)t(2;?10)(p25;q22), add(3)(q27), del(4)(q22), i(5p), i(5q), der(5)t
variable morphology; semi-adherent, spheroid to fusiform cells growing in clumps in mono- and multilayers, occasional giant cells present
Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD2 -, CD3 -, CD10 +, CD13 +, CD14 -, CD15 +, CD19 -, CD21 -, CD25 -, CD30 -, cyCD68 +, HLA-DR
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 6 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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