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GIRARDI HEART C7

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Cat.No.
CSC-C0285
Description
These cells were cloned from the GIRARDI HEART cell line (passage #623) in 1981 and were thought to "represent human heart cells"; however, isoenzyme analysis (Nelson-Rees et al., Science 191: 96-97, 1976) and DNA fingerprinting at the Creative Bioarray established unequivocally that this cell line must be considered de facto a subclone of HELA; cells were described to be sensitive to various toxins and hormones
Organism
Human
Source
cervix carcinoma
Culture Properties
monolayer
Morphology
epithelial cells growing as monolayers
Recommended Medium
45% SuperCult® Medium 199 (with Hanks salts) and 45% RPMI-1640 + 10% h.i. FBS.
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, desmin -, endothel -, GFAP -, neurofilament -, vimentin +
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV -,
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2-3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CSC-6260W
CSC-6261W
CSC-6262W
CSC-6263W
CSC-C0306
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