GIRARDI HEART C7

Cat.No.: CSC-C0285

Species: Human

Source: cervix carcinoma

Morphology: epithelial cells growing as monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C0285

Description

These cells were cloned from the GIRARDI HEART cell line (passage #623) in 1981 and were thought to "represent human heart cells"; however, isoenzyme analysis (Nelson-Rees et al., Science 191: 96-97, 1976) and DNA fingerprinting at the Creative Bioarray established unequivocally that this cell line must be considered de facto a subclone of HELA; cells were described to be sensitive to various toxins and hormones

Species

Human

Source

cervix carcinoma

Recommended Medium

45% SuperCult® Medium 199 (with Hanks salts) and 45% RPMI-1640 + 10% h.i. FBS.

Culture Properties

monolayer

Morphology

epithelial cells growing as monolayers

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, desmin -, endothel -, GFAP -, neurofilament -, vimentin +
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV -,

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2-3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What role do circulating tumor cells play in the understanding of cancer heterogeneity?

CTCs provide valuable information about the genetic and phenotypic diversity of cancer cells within a single tumor and across different metastatic sites.

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Average Rating: 4.0 | 1 Scientist has reviewed this product

Unparalleled characterization

Our experience with these cell biology products has been characterized by the unparalleled purity, functionality, and reliability that are essential for successful research outcomes.

10 June 2023

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