Allegedly established from the peripheral blood of a 40-year-old man with acute monoblastic leukemia (AML M5) at relapse in 1982; however, recent new data suggest derivation from T-ALL (acute lymphoblastic leukemia): (1) T-ALL-associated gene expression signature (Andersson et al., Leukemia 19: 1042-1050, 2005); (2) own FISH analysis confirming t(1;7) effecting TAL1-TRB@ (TAL1-TRB) fusion; (3) own reanalysis of the immunoprofile
Human near tetraploid karyotype with 9% polyploidy - 92(89-94)<4n>XX/XXY/XXYY, -1, +6, +6, -17, t(1;7)(p34.2;q34)x2, del(3)(p21), i(6q)x2, t(12;16)(q24.32;q11)x2
round or multiformed cells, singly or clustered in suspension
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD2 -, cyCD3 +, smCD3 -, CD4 -, CD5 -, CD6 (+), CD7 +, CD8 -, CD13 -, CD15 +, CD19 -, CD33 -, CD34 -, HLA-DR -, TCRalpha/beta -, TCRgamma/delta -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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