Established from peripheral blood lymphocytes of a cotton-top tamarin (cotton-top marmoset) monkey (Saguinus oedipus); cells were described to release high titers of Epstein-Barr virus (EBV) providing a source for transformation of B cells and establishment of continuous cell lines
Near-diploid simian karyotype with 4% polyploidy - 44(42-47)<2n> - chromosome rearrangements present
single, round to oval cells or clustered in small clumps in suspension and partially adherent; ca. 10% adhere, but trypsin is not necessary to detach the cells
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HIV -, HTLV-I/II -, SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 7 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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