CAL-148

Cat.No.: CSC-C0489

Species: Human

Source: breast adenocarcinoma

Morphology: epitheloid cells growing as single cells or as clumps either in suspension or, if supplemented with EGF, in monolayers

Culture Properties: monolayer

Inquire for Price

Specification
Q & A
Customer Review

Cat.No.

CSC-C0489

Description

Established from the pleural effusion from a 58-year-old French woman with terminally invasive breast cancer (moderately differentiated ductal adenocarcinoma) after repeated cycles of chemotherapy (including adriamycin and taxol)

Species

Human

Source

breast adenocarcinoma

Recommended Medium

80% DMEM + 20% h.i.FBS

Culture Properties

monolayer

Morphology

epitheloid cells growing as single cells or as clumps either in suspension or, if supplemented with EGF, in monolayers

Karyotype

Human near-diploid karyotype with 16% polyploidy - 45-48<2n>XX, +1, +7, -8, -13, +20, add(1)(p36), der(1;16)(q10;p10), del(2)(q11q2?2), del(13)(q1?1q1?3), der(16)t(1;16)(q12-21 - p11) - resembles originator's unpublished karyotype

Quality Control

Mycoplasma: negative in microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimen

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What should I do if I find a change in the original cytogenetic material of a cell in culture?

We recommend resuscitating early frozen cells for use and performing genetic identification again.

Ask a Question

Average Rating: 5.0 | 1 Scientist has reviewed this product

Good brand

Creative Bioarray is a good brand in our minds and their staff are all very responsive.

07 Sep 2021

Ease of use
After sales services
Value for money

Write your own review

You May Also Need

CM-1010X

CS-001Y

MCF7-432

Description: Species: human, Caucasian female 69 years old; Tissue: breast; Tumor: adenocarcinoma; Transfected with the exon 4 defective variant of ER-pBact cDNA, human estrogen receptor cDNA, under control of ...

Cat#: CSC-6290W INQUIRY

View Product

MCF7-492X1

Description: Species: human, Caucasian female 69 years old; Tissue: breast; Tumor: adenocarcinoma; Transfected with the pZeoSV-LacZ vector, resistant to zeocin, control for MCF7-488X1 and MCF7-490X1.; Original ...

Cat#: CSC-6293W INQUIRY

View Product

C127

Description: Host for bovine papiloma virus

Cat#: CSC-C9164W INQUIRY

View Product

EFM-192A

Description: Established from the pleural effusion of the left side from a 46-year-old Caucasian woman with metastasizing breast adenocarcinoma in 1985; sister cell lines EFM-192B and C show differences in ...

Cat#: CSC-C0358 INQUIRY

View Product

MCF7-422

Description: Species: human, Caucasian female 69 years old; Tissue: breast; Tumor: adenocarcinoma; Transfected with ER-pBact, human estrogen receptor cDNA, under control of the human beta actin promotor, ...

Cat#: CSC-6289W INQUIRY

View Product

MCF7-382

Description: Species: human, Caucasian female 69 years old; Tissue: breast; Tumor: adenocarcinoma; Transfected with pBact, expression vector under control of the human beta actin promotor, resistant to G418S.; ...

Cat#: CSC-6288W INQUIRY

View Product

For research use only. Not for any other purpose.