Established from the pleural effusion from a 58-year-old French woman with terminally invasive breast cancer (moderately differentiated ductal adenocarcinoma) after repeated cycles of chemotherapy (including adriamycin and taxol)
Human near-diploid karyotype with 16% polyploidy - 45-48<2n>XX, +1, +7, -8, -13, +20, add(1)(p36), der(1;16)(q10;p10), del(2)(q11q2?2), del(13)(q1?1q1?3), der(16)t(1;16)(q12-21 - p11) - resembles originator's unpublished karyotype
epitheloid cells growing as single cells or as clumps either in suspension or, if supplemented with EGF, in monolayers
Mycoplasma: negative in microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimen
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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