BD-215

Cat.No.: CSC-C1225

Species: Homo sapiens (Human)

Source: Blood; Peripheral Blood

Morphology: lymphoblastoid-like cells growing as single cells or in clumps in suspension

Culture Properties: suspension

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Cat.No.

CSC-C1225

Description

B lymphoblastoid cell line (B-LCL) established from the peripheral blood of a Scottish man with Fanconi anemia (complementation group C); homozygote with unrelated non-consanguineous parents; homozygous for nonsense mutation in exon 6 of the FANCC (FACC) coding sequences

Species

Homo sapiens (Human)

Source

Blood; Peripheral Blood

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

suspension

Morphology

lymphoblastoid-like cells growing as single cells or in clumps in suspension

Karyotype

Human diploid karyotype with 12% polyploidy - 46, XY, 1qh+ - no consistent abnormality detected

Disease

Fanconi Anemia

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD138 +, HLA-DR +, sm/cyIgM -, smIgG -, cyIgG +, sm/cykappa -, sm/cylambda -
Viruses: ELISA: reverse tr

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Synonyms

BD215

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.