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BD-215
- Specification
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Cat.No.
CSC-C1225
Description
B lymphoblastoid cell line (B-LCL) established from the peripheral blood of a Scottish man with Fanconi anemia (complementation group C); homozygote with unrelated non-consanguineous parents; homozygous for nonsense mutation in exon 6 of the FANCC (FACC) coding sequences
Species
Human
Source
B lymphoblastoid cells (from patient with Fanconi anemia)
Culture Properties
suspension
Morphology
lymphoblastoid-like cells growing as single cells or in clumps in suspension
Karyotype
Human diploid karyotype with 12% polyploidy - 46, XY, 1qh+ - no consistent abnormality detected
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD138 +, HLA-DR +, sm/cyIgM -, smIgG -, cyIgG +, sm/cykappa -, sm/cylambda -
Viruses: ELISA: reverse tr
Immunology: CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD138 +, HLA-DR +, sm/cyIgM -, smIgG -, cyIgG +, sm/cykappa -, sm/cylambda -
Viruses: ELISA: reverse tr
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
- Q & A
- Customer Review
Q:
Is ELISA a cell-based assay?
A:
Cell-based ELISA is a convenient, lysate-free, high throughput, and sensitive assay that can measure the relative amount of protein phosphorylation in cells. The method can also be used for monitoring the effects of various treatments, activators or inhibitors on phosphorylation.
Average Rating: 4.0 | 1 Scientist has reviewed this product
High quality
The tumor cell product was shipped at the appropriate temperature, ensuring its quality.
03 Mar 2021
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