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Immortalized Human Schwann Cells-SV40T

Cat.No.: CSC-I2111Z

Species: Homo sapiens

Morphology: Polygonal

Culture Properties: Adherent

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Cat.No.
CSC-I2111Z
Description
Immortalized Human Schwann Cells-SV40T were developed from human tissues transduced with a lentiviral expression vector containing the SV40T gene. The cell line was continuously cultured for more than 30 passages without showing signs of growth retardation or replicative senescence.
Species
Homo sapiens
Recommended Medium
Immortalized Human Schwann Cell Medium (Cat No.: CM-I2111Z)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
SV40 large T antigen
Application
For Research Use Only
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Shipping
Dry Ice.
Storage and Shipping
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Note: Never can cells be kept at -20°C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Schwann cells (SCs) play a crucial role in the development, function and regeneration of peripheral nerves. They can enhance both peripheral and central nerve regeneration by providing a supportive environment for neurite outgrowth through the release of neurotrophic factors.

Abnormalities of Schwann cells and/or their crosstalk with neurons and other non-neuronal cells in the peripheral nervous system (PNS) can lead to various types of peripheral neuropathies. Cultured Schwann cells are valuable tools for investigating the pathogenesis of peripheral neuropathies. However, use of primary SCs for in vitro models is limited because these cells are difficult to prepare and maintain in high yield and purity under common cell culture conditions.

Notably, immortalized Schwann cell lines exhibit high proliferative activity while largely retaining the distinct phenotypes of Schwann cells, making them ideal for studying pathogenic mechanisms. Unlike primary cultured cells, which have a limited lifespan, immortalized cells can be passaged indefinitely and are not subject to contact-inhibition when cultures reach confluence.

Multiomic Analyses Reveal New Targets of Polycomb Repressor Complex 2 in Schwann Lineage Cells

Malignant peripheral nerve sheath tumors (MPNSTs) can arise from atypical neurofibromas (ANF). Loss of the polycomb repressor complex 2 (PRC2) is a common event. Previous studies on PRC2-regulated genes in MPNST used genetic add-back experiments in highly aneuploid MPNST cell lines which may miss PRC2-regulated genes in NF1-mutant ANF-like precursor cells. A set of PRC2-regulated genes in human Schwann cells (SCs) has not been defined. We hypothesized that PRC2 loss has direct and indirect effects on gene expression resulting in MPNST, so we sought to identify PRC2-regulated genes in immortalized human Schwann cells (iHSCs).

We identified PRC2-regulated genes and potential drivers of MPNSTs. Our findings support the Notch pathway as a druggable target in MPNSTs. Our identification of PRC2-regulated genes and pathways could result in more novel therapeutic approaches.

PRC2 regulates Notch pathway genes in iHSCs.

Fig. 1. NOTCH signaling is a significantly upregulated pathway when PRC2 is lost in engineered iHSCs (Bhunia, Minu M., et al. 2024).
What are Immortalized Human Schwann Cells-SV40T?

Immortalized Human Schwann Cells-SV40T are human Schwann cells that have been genetically modified with the SV40 large T antigen to enable continuous growth and division. This immortalization process allows for extended research use and reliable data generation.

What research applications are ideal for using these cells?

These cells are ideally suited for research in neuroscience, particularly in studies of nerve regeneration and peripheral neuropathies. They provide an excellent model for testing therapeutic compounds, exploring myelination processes, and conducting neurotoxicology assessments.

How do Immortalized Human Schwann Cells (SV40T) benefit my research?

These cells offer consistent, long-term usability without the drawbacks of primary cell culture, such as limited life span and variability. Their immortalized nature facilitates reproducibility and scalability in experiments, critical for robust scientific research.

Are these cells suitable for drug screening applications?

Yes, the uniformity and reproducibility of these cells make them highly suitable for drug screening and testing, particularly for compounds targeting nerve repair and neuroprotection, enhancing the discovery of effective therapies.

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For research use only. Not for any other purpose.