Human iPSC-derived Osteoblasts

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Cat.No.

CSC-00839L

Description

Osteoblasts synthesize collagen and express specialized proteins such as osteoclacin and osteopontin. As an organized group of connected cells, osteoblasts form part of the mineralized matrix. The matrix is part of the crucial skeletal support for humans. Mineralization is part of human's physiological homeostasis, such as acid-base balance, calcium or potassium maintenance. Human iPSC-derived Osteoblasts serve as a model system to examine bone mineralization, matrix formation for skeletal systems, bone remodeling, and bone metabolism. Human iPSC-derived Osteoblasts are reprogrammed using our proprietary serum-free, virus-free, nucleic-acids-free, feeder-free, and integration-free technology.. They expression biomarkers: Osteocalcin, COL1A1, FLT1, Bone Sialoprotein, RUNX2, and SPP1 (q/RT-PCR data not shown). Bone mineralization data (Alizarin S Red; 530nm absorbance data) are shown.

Species

Human

Application

For Research Use Only

Shipping

Dry Ice

Quality Control

Sterility, Safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>92%)

Storage and Shipping

Remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Human iPSC-derived Osteoblasts (hiPSC-OBs) are osteoblasts that are created by the directed differentiation of human induced Pluripotent Stem Cells (hiPSCs). iPSCs are stem cells that have been reprogrammed from adult somatic cells, such as dermal fibroblasts or peripheral blood cells. The differentiation process recapitulates osteogenesis in vivo. This begins with the induction of hiPSCs to become mesenchymal stem cells (MSCs) by activating BMP4 or TGF-β signaling and osteogenic commitment by stimulating the Wnt/β-catenin pathway and adding osteogenic supplements (ascorbic acid, β-glycerophosphate, dexamethasone), which induces osteoblast maturation. As they mature, they aggregate into mineralized nodules-hallmarks of osteoblastic function-while maintaining a stable karyotype and osteogenic lineage commitment.

Functionally, hiPSC-OBs possess core osteoblastic properties: extracellular matrix (ECM) synthesis (collagen type I, osteocalcin) and matrix mineralization, critical for bone formation. These traits make them pivotal in multiple fields. In basic science, hiPSC-OBs allow for the recapitulation of human osteogenesis in vitro, allowing for the study of mechanisms and signals of osteogenic lineage specification and mineralization that cannot be gleaned in animal models. In drug discovery, they can be used as a platform for screening of osteoprotective small molecules and chemicals in a human-relevant context, rather than in animal models that are often used as first line screening methods. They are also important in the field of regenerative medicine; preclinical models have demonstrated their usefulness in bone tissue engineering-seeding hiPSC-OBs on scaffolds allowed for the repair of bone defects in cases of fracture and craniofacial abnormalities.

Cellular Behavior of hiPSC-Derived Osteoblasts on a Bone-Mimetic Collagen Scaffold

hiPSC-derived osteoblasts (hiPSC-Obs) have shown preferential alignment and organization in bone-mimetic collagen scaffolds, highlighting their role in unidirectional cellular arrangement. However, the mechanisms underlying their oriented tissue construction properties remain unclear. Matsugaki et al. aim to characterize the properties of hiPSC-Obs and their focal adhesions (FAs) to understand their role in anisotropic bone microstructural regeneration.

The cellular properties and focal adhesions (FAs) of hiPSC-Obs were characterized by analyzing their behavior on a bone-mimetic collagen scaffold (Fig. 1 and Fig. 2). On polystyrene plates, both hiPSC-Obs and NHObs exhibited non-uniform shapes and random orientations (Fig. 1A). On the collagen scaffold, both types of osteoblasts showed elongated bodies and aligned predominantly along the collagen fibers (Fig.1B). The collagen scaffold promoted preferential cellular orientation. Notably, hiPSC-Obs showed better responsiveness than NHObs, with decreased deviation of the cellular orientation angle (Fig. 1B and Fig. 2A) and increased cell proliferation (Fig. 2B). Additionally, hiPSC-Obs exhibited an ordered array of FAs parallel to the cell orientation axis (Fig. 1B and Fig. 2C) and an increased number of FAs (Fig. 1B and Fig. 2D).

Observation of the cell behavior of human-induced pluripotent stem cell-derived osteoblasts (hiPSC-Obs) on a bone-mimetic collagen scaffold. — Fig. 1. Observation of the cell behavior of human-induced pluripotent stem cell-derived osteoblasts (hiPSC-Obs) on a bone-mimetic collagen scaffold (Ozasa R, Matsugaki A, *et al.*, 2021).

Estimation of the properties of cells and focal adhesions (FAs). — Fig. 2. Estimation of the properties of cells and focal adhesions (FAs) (Ozasa R, Matsugaki A, *et al.*, 2021).

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For research use only. Not for any other purpose.