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Immortalized Rat Steroidogenic Granulosa Cells stably expressing LH/CG Receptor (GLHR-15)
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Cat.No.
CSC-I9174L
Description
Lutropin/choriogonadotropin (LH/CG) receptor, a receptor uniquely expressed in ovarian granulosa and testicular sertoli cells, play an important role in the regulation of oogenesis, spermatogenesis and steroid hormone production. Unfortunately the LH/CG receptor expression is often lost within 1-2 days ofin vitroculture of primary cells.The Immortalized Rat Steroidogenic Granulosa Cells stably expressing LH/CG Receptor is responsive to luteinizing hormone (LH) or choriogonadtropin (hCG) stimulation and produces progesterone and 20α-OH-P as a result at levels comparable to primary cells. This cell line is useful in studying molecular mechanisms associated with gonadotropin stimulated steroidogenesis. Together with theImmortalized Rat Steroidogenic Granulosa Cells expressing FSH Receptor, researchers can compare the LH and FSH signaling in homologues cell systems.
Source
Ovary
Culture Properties
Adherent
Immortalization Method
Serial passaging and calcium phosphate mediated cotransfection by SV40 DNA and Ha-ras oncogene
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
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Quality Control
1) Phenotypic response to hCG stimulation was characterized by shape changes (cells rounding up as a result of intracellular cAMP accumulation) ;
2) LH/CG receptor expression was measured by radiolabelled 125I-hCG binding assay- the cells express 19,000 FSH receptors/cell with a Kd of 180 pM, similar to the native receptor;
3) Production of progesterone and 20α-OH-P was determined by radioimmunoassay (RIA) ;
4) Production of cAMP was measured by a protein binding method.
2) LH/CG receptor expression was measured by radiolabelled 125I-hCG binding assay- the cells express 19,000 FSH receptors/cell with a Kd of 180 pM, similar to the native receptor;
3) Production of progesterone and 20α-OH-P was determined by radioimmunoassay (RIA) ;
4) Production of cAMP was measured by a protein binding method.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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