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Immortalized Mouse Ovarian Surface Epithelial Cells (iMOSEC)
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Cat.No.
CSC-I9184L
Description
The Immortalized Ovarian Surface Epithelial Cells (iMOSEC) is derived by co-expression of adenovirus E1A and dominant-negative p53 (p53DD). In addition to its non-tumorigenicity, this cell line maintains its primary phenotype and expresses epithelial-specific markers such as E-cadherin, cytokeratin 8 and 18. This cell line is a useful model for studying ovary growth regulation.
Species
Mus musculus
Source
Ovarian surface
Culture Properties
Adherent
Morphology
Cuboidal
Immortalization Method
Serial passaging and electroporation transfection with linearized DNA of E1A and p53DD
Markers
E-cadherin, cytokeratin 8 and cytokeratin 18
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
1. Western blot was used to confirm the presence of E1A and p53DD gene in the immortalized cell line.
2. Western blot was used to check epithelial markers E-cadherin and cytokeratin expression.
2. Western blot was used to check epithelial markers E-cadherin and cytokeratin expression.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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