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Immortalized Mouse Mammary Epithelial Cells (iMMEC)
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Cat.No.
CSC-I9180L
Description
The Immortalized Mouse Mammary Epithelial Cells (iMMEC) is derived by co-expression of adenovirus E1A and dominant-negative p53 (p53DD). This cell line is unique in that 1) it expresses surface markers, including estrogen-receptor alpha (ERα) and retains the functional properties characteristic to mammary epithelial cell and 2) upon lactogenic stimulation it forms dome-like structures indicative of mammary alveoli and secrets β-catenin into the acinar lumen. This cell line is suitable for studies in hormone regulation and 3D dimensional ductal morphogenesis.
Species
Mus musculus
Source
Mammary Epithelia
Culture Properties
Adherent
Morphology
Cuboidal
Immortalization Method
Serial passaging and electroporation transfection with linearized DNA of E1A and p53DD
Markers
β-catenin, E-cadherin, Ep-CAM, CK8 and CK18
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
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Quality Control
1. Western blot was used to confirm the presence of E1A and p53DD gene in the immortalized cell line.
2. Western blot and immunofluorescence were used to check gene expression of β-catenin, E-cadherin, Ep-CAM, CK8, CK18 and ERα.
2. Western blot and immunofluorescence were used to check gene expression of β-catenin, E-cadherin, Ep-CAM, CK8, CK18 and ERα.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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