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Immortalized Mouse Lung Epithelial Cells (MLE-15)
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Cat.No.
CSC-I9200L
Description
Nonciliated bronchiolar (Clara) cells, alveolar type I and type II cells are the predominant cell types in the respiratory epithelium. Clara cells and alveolar type II cells produce pulmonary surfactant proteins that are critical in the structure and function maintenance of the pulmonary alveolus by reducing surface tension at the air-liquid interface. The Immortalized Mouse Lung Epithelial Cells (MLE-15) is a cell line derived from transgenic FVB/N mice harbouring SV40 TAg under the control of the human SP-C promoter. Similar to alveolar type II cells, MLE-15 is characterized by the presence of apical microvilli and intracellular multiamellar inclusion bodies, as well as the ability to produce surfactant proteins B and C. This cell line is useful in studying the regulation of lung epithelial genes and elucidating the complex transcriptional networks related to airway/alveolar differentiation.
Species
Mus musculus
Source
Distal respiratory epithelium
Culture Properties
Adherent
Morphology
Polygonal
Markers
SP-A (low level), SP-B, SP-C
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
1) Electron microscopy was used to evaluate the presence of microvilli and lamellar bodies;
2) in situ hybridization was performed to assess the presence of respiratory epithelial cell markers;
3) Immunoprecipitation was performed to determine the secretion of surfactant proteins;
4) Northern blot was used to detect SP-A, SP-B and SP-C, and SV40 TAg mRNA.
2) in situ hybridization was performed to assess the presence of respiratory epithelial cell markers;
3) Immunoprecipitation was performed to determine the secretion of surfactant proteins;
4) Northern blot was used to detect SP-A, SP-B and SP-C, and SV40 TAg mRNA.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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