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Immortalized Mouse Distal Caput Epididymal Epithelial Cell Line (DC2)
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Cat.No.
CSC-I9202L
Description
The epididymal epithelium plays an important role in male fertility by supporting sperm motility, maturation and viability. Owing to the changing microenvironment created by different sections of the epididymis, the spermatozoa matures as they passage through the convoluted tubule.DC2 is a conditionally immortalized mouse epididymal epithelial cell line isolated from the distal caput of the mouse harbouring a temperature-sensitive simian virus 40 large T antigen. The functional expression of the SV40 large T antigen is induced by culturing the cellsin vitroat permissive temperature (33°C). At a non-permissive temperature (37°C-39°C), the cells cease to proliferate. DC2 expresses androgen receptor as well as markers of the murine epididymal epithelium, PEB-like protein (i.e. phosphatidylethanolamine binding protein), E-RABP (i.e. epididymal retinoic acid-binding protein), and EP17 (i.e. epididymal protein of 17 kd). Together with the Immortalized Mouse Proximal Caput Epididymal Epithelial Cell Line (PC1), these cells are useful in studying the regulation of tissue-specific gene expression and epididymal specific proteins in the epididymis.
Species
Mus musculus
Source
caput epididymis
Culture Properties
Adherent
Morphology
polygonal
Immortalization Method
Isolated from a transgenice mouse carrying a temperature-sensitive simian virus 40 large T tumor antigen
Markers
Androgen receptor (AR), PEB-like protein, E-RABP, mEP17, cytokeratin
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
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CIK-HT004 HT® Lenti-SV40 (tsA58 temperature sensitive mutant) Lentivirus Immortalization Kit
Quality Control
1) Immunocytochemistry, western blot and RT-PCR were used to confirm expression of androgen receptor, cytokeratin, mE-RABP, PEB-like protein, and mEP17;
2) RT-PCR was used to assess the T-antigen transgene expression in the immortalized cell line.
2) RT-PCR was used to assess the T-antigen transgene expression in the immortalized cell line.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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