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Immortalized Mouse Dendritic Cells (MutuDC1940)
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Cat.No.
CSC-I9098L
Description
As messengers bridging the innate and adaptive immune system, the conventional tissue-resident DC (cDC) acts as sentinels in secondary lymphoid organs and other tissues for antigen capture and presentation. The Wild-type MutuDC1940 cell line is an immortalized splenic CD8α+subset (CD11chigh,B220-,DEC205+,CD24high,CD11b-) of conventional dendritic cells derived from the CD11c:SV40LgT transgenic mice.The MutuDC1940 cell line retains response to TLR ligands such as CpG (TLR9-L) and PolyIC (TLR3-L) and to a lesser extent LPS (TLR4-L) stimulation by up-regulation of co-stimulatory molecules CD40, CD80 and CD86. In addition to responding to PAMP stimulation and producing Th1 cytokines such as IL-12, these cells are also capable of presenting antigen in the context of both MHC-I and MHC-II, including direct antigen presentation and cross-presentation of cell-associated antigens. Together with TLR3 knockout, TLR9 knockout and Ifnar1 knockout MutuDC, these dendritic cell lines provide a powerful tool in vaccine science and immunotherapy, particularly in strategizing target antigens to CD8α+subset.
Species
Mus musculus
Source
Spleen
Culture Properties
Adherent
Morphology
Small Aggregates
Immortalization Method
Isolated from C57BL/6 transgenic mice carrying SV40 Large T oncogene
Markers
CD11c, CD24, MHC-II+, DEC205, Clec9A, B220, CD11b, IRF4, IRF8, CD4
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
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Quality Control
1) Direct antigen presentation and cross-antigen presentation were evaluated by the MHC-I (SIINFEKL/OT-I ) and MHC-II (OVA323-339/OT-II) restricted systems;
2) proteome profile and surface markers assessed by RT-PCR; RT-PCR;
3) IL-12 cytokine secretion analyzed using ELISA;
4) Response to PAMP stimulation evaluated by functional assays.
2) proteome profile and surface markers assessed by RT-PCR; RT-PCR;
3) IL-12 cytokine secretion analyzed using ELISA;
4) Response to PAMP stimulation evaluated by functional assays.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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