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Immortalized Mouse Cortical Collecting Duct Cell Line (mCCD(cl1))
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Cat.No.
CSC-I9092L
Description
In aldosterone-sensitive distal nephron (ASDN), mineralocorticoid receptor (MR) and glucocorticoid receptor (GR) activation regulate ionic and water transports through the epithelial sodium channel (ENaC) and the Na+/ K+ pump. The Immortalized Mouse Cortical Collecting Duct Cell Line is a spontaneously transformed Liddle mouse cell line that expresses significant level of functionally active MR and GR, as well as active 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2) enzyme. In addition, this cell line responses to physiological concentrations (K(1/2)= 0.3 nM) of aldosterone, making it useful for binding equilibrium assays and studies on sodium transport response to various mineralocorticoid and glucocorticoid agonists.
Species
Mus musculus
Source
Kidney
Culture Properties
Adherent
Immortalization Method
Spontaneously immortalization
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
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Quality Control
1) Immunofluorescence and RT-PCR were used to confirm the expression of epithelial sodium channel (ENaC) and Na-K-ATPase;
2) RT-PCR was used to detect expression of MR, GR, and 11β-HSD2;
3) sodium transport response assayed through short-circuit current (electrophysiologic studies) and binding assays.
2) RT-PCR was used to detect expression of MR, GR, and 11β-HSD2;
3) sodium transport response assayed through short-circuit current (electrophysiologic studies) and binding assays.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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