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Immortalized Mouse Cardiomyogenic Progenitor Cells (iCP)
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Cat.No.
CSC-I9226L
Description
The Immortalized Mouse Cardiomyogenic Progenitor Cells are hygromycin-resistant, non-tumorigenic reversibly immortalized cells that have cardiomyocyte-like morphology and have maintained rhythmatic beating in culture. It expresses late stem cell markers (Oct3/4, Nanog, Sox2), c-kit, early cardiomyogenic markers (ISL-1, Hand2, Sca-1), and cardiomyocyte-specific markers (α-actin and α-myosin heavy chain). The immortalization process may be reversed by infection with Cre-recombinase expressing adenoviruses to revert immortal cells back into primary cells resulting in increasing differentiation potential. In the presence of retinoic acid, the cells undergo cardiomyogenesis where late cardiomyogenic markers (Actc1 and ANF) will be present. Dexamethasone may be used as a differentiation reagent, to express late markers ANF, cTnT, and α-MyHC. These cells are recommended for studies relating to cardiomyogenic progenitors in basic and/or translational research.
Species
Mus musculus
Source
Heart
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
Serial passaging and transduction with retrovirus SSR#69 expressing SV40 large T antigen flanked with Cre/loxP sites
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
1) RT-PCR, qPCR, and sqPCR to detect cell markers;
2) Western blot analysis for SV40 large T antigen presence.
2) Western blot analysis for SV40 large T antigen presence.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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