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Immortalized Mouse Astrocytes-SV40T (IMA2.1)
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Cat.No.
CSC-I9175L
Description
The Immortalized Mouse Astrocytes- SV40 T (IMA 2.1) is a suitable cell line for studies in astrocyte functions. This cell line is unique in that it is capable of metabolizing the parkinsonian toxin MPTP (to MPP+) and is responsive to pro-inflammatory stimuli. In addition, this cell line can be used in neuronal co-culture studies to simulate conditions in the brain.Upon stimulation with cytokines such as IL-1β, TNF-α and IFN-γ, these cells show up-regulated NOS-2 and COX-2 mRNA expression and release various inflammatory mediators. This cell line has a stable star-shape morphology and expresses immature astrocyte markers such as nestin and CD44, indicating that the cells are of the early-stage astrocyte precursor phenotype. Furthermore, they are capable of converting glutamate via glutamine synthase to glutamine, which is a characteristic of GFAP-negative astrocytes.
Species
Mus musculus
Source
Cerebral Cortex
Culture Properties
Adherent
Morphology
star-shape morphology
Immortalization Method
Serial passaging and transfection with psV3neo plasmid carrying SV40 large T antigen genes
Markers
Nestin, CD44
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
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Quality Control
The cell line's response to pro-inflammatory stimuli was measured by
1) immunostaining to assess NF-kB translocation from the cytosol to the nucleus;
2) RT-qPCR and quantitative PCR detection of NOS-2 mRNA levels;
3) Western Blot analysis of NOS-2 and COX2 proteins.
1) immunostaining to assess NF-kB translocation from the cytosol to the nucleus;
2) RT-qPCR and quantitative PCR detection of NOS-2 mRNA levels;
3) Western Blot analysis of NOS-2 and COX2 proteins.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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