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Immortalized (Conditionally) Mouse Pancreas Epithelial Cells (IMPE)
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Cat.No.
CSC-I9189L
Description
IMPE is a conditionally immortalized mouse pancreas epithelial cell line isolated from the mouse harboring thermolabile mutation (tsA58) under the control of an interferon (IFN)-γ-inducible H-2Kbpromoter and a temperature-sensitive simian virus 40 large T antigen. The functional expression of the SV40 large T antigen is induced by culturing the cellsin vitroin medium containing IFN γ at a temperature permissive (33°C). At a non-permissive temperature (37°C-39°C) or when cultured without IFN γ over 3 passages, the cells cease to proliferate. The IMPE has been shown to produce insulin after transfection with pdx1 and culture in the presence of glucagon-like peptide 1 (GLP1).
Species
Mus musculus
Source
Pancreas
Culture Properties
Adherent
Morphology
Cobblestone-like
Immortalization Method
Isolated from transgenic mouse carrying a temperature-sensitive simian virus 40 tumor antigen (tsA58)
Markers
β-catenin, E-Cadherin, CK8, CK18, CK19
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
1) Western Blot and immunofluorescence were used to detect the presence of SV40 large T antigen;
2) Western Blot was utilized to detect the expression of epithelial markers E-Cadherin and β-catenin;
3) RT-PCR was used to verify the expression of CK8, CK18 and CK19.
2) Western Blot was utilized to detect the expression of epithelial markers E-Cadherin and β-catenin;
3) RT-PCR was used to verify the expression of CK8, CK18 and CK19.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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