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SuperCult® Embryoid Body (EB) Formation Medium

  • Specification
  • Recommended Products
Cat.No.
CM-1282W
Description
Embryonic stem cells (ESCs), derived from the inner cell mass (ICM) of blastocyst-stage embryos, are pluripotent and have a virtually unlimited capacity of self-renewal. They can differentiate into all cell types comprised of all three embryonic germ layers (ectoderm, mesoderm and endoderm). The formation of embryoid bodies (EBs) is a principal step in the differentiation of ESCs. When maintained in the EB formation medium and in the absence of MEF feeder layers, ES cells differentiate spontaneously and form three-dimensional aggregates. This structure facilitates multicellular interactions in which cell-cell contact occurs and gap junctions are established.
Quality Control
Tested for perfomance on EB formation. The standard evaluation includes: 1. Sterility test (bacteria, fungi and mycoplasma) 2. pH test 3. Osmolality 4. Endotoxin
Storage and Shipping
- All products should be stored in the dark. - Embryoid Body Formation Basal Medium is stable at 2-8 °C for up to one year; other components are stable at -20 °C for up to two years. - Products should be discarded beyond the labeled expiration date. - Once prepared, the fully supplemented (complete) medium can be stored for up to one month when stored in the dark at 2-8 °C. - For optimal performance, repeated warm-cooling and freeze-thawing should be avoided.
Components
Embryoid Body (EB) Formation Basal Medium:435 mL Embryoid Body (EB) Formation- Qualified Fetal Bovine Serum:50 mL Penicillin-Streptomycin:5 mL Glutamine:5 mL Non-Essential Amino Acid:5 mL 2-Mercaptoethanol:500 ul
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
Customer Support & Price Inquiry
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CONTACT US USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
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Europe
Tel: 44-207-097-1828
Email: