DLD-1

The Effect of the Cardaria draba Subspecies Shalepensis Exerts on the Cell Viability of the Human Colon Cancer Cell Line (DLD-1)

Metabolic dysregulation and aberrant melanogenesis lead to hyperglycaemia and hyperpigmentation, respectively. Conventional treatments are often suboptimal. Ortaakarsu et al. investigated the inhibitory effects of Cardaria draba extract on α-glucosidase and tyrosinase-related protein 1, and its antiproliferative effects on DLD-1 colon cancer cells, to explore its potential as a therapeutic agent.

The cytotoxicity effect of the plant extract on Human Colon Cancer Cell Line (DLD-1) was evaluated by MTT method (Fig. 1). The viability values of DLD-1 cells were found to be 22.23% at 312 µg/mL and 38.94% at 625 µg/mL. The viability of cells at 1250 µg/mL was 87.61% and it decreased to 80.26% at 2500 µg/mL. The viability at 1250 and 2500 µg/mL increased, while at 312 and 625 µg/mL it decreased. Thus, the most suitable concentrations for DLD-1 were determined as 312 and 625 µg/mL. The IC₅₀ value of DLD-1 cell line was found to be 767 µg/mL. ANOVA with Bonferroni corrections showed that the extract had significant effect. The change in trend at 2500 µg/mL was explained by the exceeding of threshold concentrations by the compounds of the extract, which causes a disturbance in the mechanisms that form the defense of the cell line.