DLD-1

Specification
Recommended Products

Cat.No.

CSC-C9371L

Description

DLD-1 is one of two colorectal adenocarcinoma cell lines which were isolated by D.L. Dexter and associates during a period from 1977-1979. The cells have been used in the study of polar solvents on cell characteristics. The four cell lines: DLD-1, HCT-15, HCT-8 and HRT-18 have been shown to have a common genetic origin. DLD-1 can express carcinoembryonic antigen (CEA) 0.5 ng/106 cells/10 days and colon antigen 3. These cells are weakly positive for keratins and vimentin. The cells are positive for keratin by immunoperoxidase staining.

Species

Homo sapiens, human

Source

Colon

Recommended Medium

90% RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3 + 10% h.i. FBS

Culture Properties

monolayer

Morphology

Epithelial

STR DNA Profile

Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,11
D16S539: 12,13
D5S818: 13
D7S820: 10,12
THO1: 7,9.3
TPOX: 8,11
vWA: 18,19

Karyotype

This is a pseudodiploid human cell line with the modal chromosome number of 46, occurring in 86% of cells. The rate of polyploidy was high at 17.1%. The karyotype of the line was 46,XY,-2,+dir dup(2)(p13-p23). The Y chromosome was slightly longer than N22 and had a large segment of heterochromatic, fluorescent distal q arms.

Applications

This cell line can be used as a suitable transfection host.

Disease

Colon adenocarcinoma

Quality Control

Tests for mycoplasma, bacteria and fungi were negative

Storage and Shipping

Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

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CM-1002X

SuperCult® RPMI-1640 with L-glutamine & HEPES

INQUIRY

CS-001Y

PremSera® FBS

INQUIRY

For research use only. Not for any other purpose.