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DLD-1

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Cat.No.
CSC-C9371L
Description
DLD-1 is one of two colorectal adenocarcinoma cell lines which were isolated by D.L. Dexter and associates during a period from 1977-1979. The cells have been used in the study of polar solvents on cell characteristics. The four cell lines: DLD-1, HCT-15, HCT-8 and HRT-18 have been shown to have a common genetic origin. DLD-1 can express carcinoembryonic antigen (CEA) 0.5 ng/106 cells/10 days and colon antigen 3. These cells are weakly positive for keratins and vimentin. The cells are positive for keratin by immunoperoxidase staining.
Organism
Homo sapiens, human
STR DNA Profile
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,11
D16S539: 12,13
D5S818: 13
D7S820: 10,12
THO1: 7,9.3
TPOX: 8,11
vWA: 18,19
Karyotype
This is a pseudodiploid human cell line with the modal chromosome number of 46, occurring in 86% of cells. The rate of polyploidy was high at 17.1%. The karyotype of the line was 46,XY,-2,+dir dup(2)(p13-p23). The Y chromosome was slightly longer than N22 and had a large segment of heterochromatic, fluorescent distal q arms.
Source
Colon
Culture Properties
monolayer
Morphology
Epithelial
Quality Control
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Disease
Colon adenocarcinoma
Application
This cell line can be used as a suitable transfection host.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CSC-6317W
CSC-C0204
CSC-C0253
CSC-C0256
CSC-C0300
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