DLD-1 is one of two colorectal adenocarcinoma cell lines which were isolated by D.L. Dexter and associates during a period from 1977-1979. The cells have been used in the study of polar solvents on cell characteristics. The four cell lines: DLD-1, HCT-15, HCT-8 and HRT-18 have been shown to have a common genetic origin. DLD-1 can express carcinoembryonic antigen (CEA) 0.5 ng/106 cells/10 days and colon antigen 3. These cells are weakly positive for keratins and vimentin. The cells are positive for keratin by immunoperoxidase staining.
Homo sapiens, human
STR DNA Profile
This is a pseudodiploid human cell line with the modal chromosome number of 46, occurring in 86% of cells. The rate of polyploidy was high at 17.1%. The karyotype of the line was 46,XY,-2,+dir dup(2)(p13-p23). The Y chromosome was slightly longer than N22 and had a large segment of heterochromatic, fluorescent distal q arms.
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
This cell line can be used as a suitable transfection host.
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