CMK

Cat.No.: CSC-C0439

Species: Human

Source: acute megakaryocytic leukemia

Morphology: single, multiformed, large cells growing in suspension, sometimes loosely adherent

Culture Properties: suspension

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Cat.No.

CSC-C0439

Description

Established from the peripheral blood of a 10-month-old boy with Downs syndrome and acute megakaryocytic leukemia (AML M7) at relapse in 1985

Species

Human

Source

acute megakaryocytic leukemia

Recommended Medium

80% RPMI-1640 + 20% h.i. FBS

Culture Properties

suspension

Morphology

single, multiformed, large cells growing in suspension, sometimes loosely adherent

Karyotype

Human flat-moded hypotetraploid karyotype with 8% polyploidy - 85-90<4n>XY, -X, -Y, -2, -3, +5, -6, -6, -8, +11, -15, -15, +16, -17, -19, +21, +22, +7-11mar, add(1)(q31), add(1)(p36), add(3)(q11), del(3)(p14)x2-3, add(5)(q11), add(5)(q13), dup(8)(q11q21),

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 +, CD41 +, CD71 +, CD235a +
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

How does fluorescence in situ hybridization work?

In this technique, the full set of chromosomes from an individual is affixed to a glass slide and then exposed to a “probe”—a small piece of purified DNA tagged with a fluorescent dye. The fluorescently labeled probe finds and then binds to its matching sequence within the set of chromosomes.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

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We use lymphoma cell products in vitro trials to evaluate potential therapeutic agents for lymphoma treatment. Creative Bioarray products have played a major role in our experiments.

10 Oct 2022

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For research use only. Not for any other purpose.