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Cat.No.
CSC-C0439
Description
Established from the peripheral blood of a 10-month-old boy with Downs syndrome and acute megakaryocytic leukemia (AML M7) at relapse in 1985
Species
Human
Source
acute megakaryocytic leukemia
Recommended Medium
Culture Properties
suspension
Morphology
single, multiformed, large cells growing in suspension, sometimes loosely adherent
Karyotype
Human flat-moded hypotetraploid karyotype with 8% polyploidy - 85-90<4n>XY, -X, -Y, -2, -3, +5, -6, -6, -8, +11, -15, -15, +16, -17, -19, +21, +22, +7-11mar, add(1)(q31), add(1)(p36), add(3)(q11), del(3)(p14)x2-3, add(5)(q11), add(5)(q13), dup(8)(q11q21),
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 +, CD41 +, CD71 +, CD235a +
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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Q:
How does fluorescence in situ hybridization work?
A:

In this technique, the full set of chromosomes from an individual is affixed to a glass slide and then exposed to a “probe”—a small piece of purified DNA tagged with a fluorescent dye. The fluorescently labeled probe finds and then binds to its matching sequence within the set of chromosomes.

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Average Rating: 5.0    |    1 Scientist has reviewed this product

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We use lymphoma cell products in vitro trials to evaluate potential therapeutic agents for lymphoma treatment. Creative Bioarray products have played a major role in our experiments.

10 Oct 2022


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