ActoFactor™ Recombinant Mouse Sonic hedgehog

CSC-CTK0774

Recombinant mouse Sonic Hedgehog is part of a small group of secreted proteins that are vital for development in both vertebrates and invertebrates. Three mammalian hedgehog genes (sonic, desert, Indian) share about 60% homology. The mouse Sonic Hedgehog is 99% homologous to the human gene. Sonic Hedgehog is a protein that is vital in guiding the early embryo. It has been associated as the major inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. It binds to the patched receptor, which functions in association with smoothened, to activate the transcription of target genes. In the absence of sonic Hedgehog, patched receptor represses the constitutive signaling activity of smoothened. Sonic Hedgehog also regulates another factor, the gli oncogene. Sonic Hedgehog intercellular signal is essential for a various patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Sonic Hedgehog exhibits both floor plate- and motor neuron-inducing activity. Mutations in a long- range Sonic Hedgehog enhancer located in an intron of the limb region 1 gene result in preaxial polydactyly. Recombinant mouse Sonic Hedgehog produced in E. coli is a single, non-glycosylated polypeptide chain containing 176 amino acids and having a molecular mass of 19.8 kDa. Cysteine at position 25 has been substituted with Ile.

Mouse

Mouse Shh expressed in E.coli

19.8 kDa

CAT# CSC-CTK0774-25 (25 μg); CAT# CSC-CTK0774-125 (125 μg)

E.coli

Greater than 97% as determined by SDS-PAGE and RP-HPLC analysis.

Determined by it's ability to induce alkaline phosphatase production by C3H10T1/2 fibroblasts, with an expected ED50 in the range of 0.05 – 0.25 μg/ml.

N/A

Sterile-filtered, white, lyophilized (freeze-dried) powder. Lyophilized from a protein solution in 10 mM sodium phosphate (pH 7.5).

Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze-thaw cycles.

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