Macrophage-colony stimulating factor (M-CSF or CSF-1) can be produced by a number of cells including fibroblasts, activated macrophages, secretory epithelial cells of the endometrium, bone marrow stromal cells, brain astrocytes, osteoblasts, renal mesangial cells, keratinocytes and LPS- or cytokine-activated endothelial cells. M-CSF stimulates the formation of macrophage colonies from bone marrow hematopoietic progenitor cells. It also induces proliferation of isolated macrophages, enhances antibody-dependent cell-mediated cytotoxicity, primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages and stimulates pinocytosis and osteoclast differentiation. M-CSF is synthesized as a membrane-bound propeptide. After proteolytic processing, M-CSF can be secreted in its soluble form or anchored to the membrane. Both forms are biologically active. Natural M-CSFs are glycosylated, disulfide-linked, homodimeric proteins with molecular weights ranging from 40 - 70 kDa. The carbohydrate moiety is not necessary for biological activity. Several C-terminal variants of the soluble form of M-CSF have been found. The N-terminal of mature M-CSF contains 150 amino acids and is necessary and sufficient for interaction with the M-CSF receptor. The human and mouse forms of the N-terminal are highly conserved (80% homology).
Mouse Csf1 expressed in E.coli
A DNA sequence encoding the N-terminal 230 amino acid residues of the extracellular domain of the mature mouse M-CSF protein sequence (residues 33 - 262 of the mouse M-CSF precursor) was expressed in E. coli.
CAT# CSC-CTK0084-10 (10 μg); CAT# CSC-CTK0084-50 (50 μg)
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
The biological activity of recombinant mouse M-CSF is measured in a cell proliferation assay using an M-CSF-dependent murine monocytic cell line, M-NFS-60. The ED50 for this effect is typically 0.5 - 1.5 ng/mL.
Endotoxin level is <1.0 EU per 1 μg cytokine, as determined by the LAL method.
Recombinant mouse M-CSF is lyophilized from a 0.2 μm filtered solution in phosphate buffered saline (PBS) containing 50 μg bovine serum albumin per 1 μg cytokine.
Reconstitute mouse M-CSF at a concentration greater than 10 μg/mL with sterile PBS containing at least 0.1% human or bovine serum albumin.
Storage & Stability
Lyophilized mouse M-CSF is stable for upto twelve months from date of receipt at -20°C to -70°C. Reconstituted mouse M-CSF can be stored under sterile conditions at 2°C - 8°C for one month, or at -20°C to -70°C (in a manual defrost freezer) for three months without detectable loss of activity. Avoid repeated freezing and thawing.
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