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ECC4
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Store in liquid nitrogen.
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Before the experiment starts, put the 15 mL centrifuge tube, pipette, gun tip and other consumables needed for the experiment into the sterile ultra-clean bench and irradiate with UV light for 30min. preheat the complete culture medium, PBS and trypsin to 37℃. Observe the cell morphology and growth density under an inverted microscope. When the cell growth density reaches 80%-90% confluence, the cells can be passaged.
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01 Oct 2022
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