DOHH-2

Specification
Technical Resources
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Cat.No.

CSC-C0219

Description

Established from the pleural effusion of a 60-year-old man with refractory immunoblastic B cell lymphoma progressed from follicular centroblastic/centrocytic lymphoma in 1990; cells were described to carry the t(14;18) leading to IGH@-BCL2 (IGH-BCL2) fusion gene and to express BCL2 mRNA; the original culture was a mixture of EBV - and EBV + cells, of which the EBV - cells were isolated and clonally expanded

Species

Human

Source

B cell lymphoma

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

suspension

Morphology

cells grow as single cells or in clumps in suspension

Karyotype

Human hyperdiploid karyotype - 47(43-48)<2n>XY, +7, der(8)t(8;18)(q24;q21), der(14)t(8;14)(q24;q32), der(18)t(14;18)(q32;q21) - earliest of a recent series of cell lines with cohabiting 8;14 (Burkitt or diffuse large cell lymphoma) and 14;18 (follicular l

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa -, sm/cylambda +
Viruses: ELISA:

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 8 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Product Sheets

Datasheet

No 'Material Safety Data Sheet' available yet

CM-1448Y

SuperCult® RPMI 1640 Medium

INQUIRY

CS-001Y

PremSera® FBS

INQUIRY

For research use only. Not for any other purpose.

Q & A
Customer Review
Product Information Sheet

What happens in G1 phase?

During the G1 phase, cells accomplish most of their growth. They get bigger in size and make proteins and organelles needed for normal functions of DNA synthesis. Here, proteins and RNAs are synthesized, and, more especially the centromere and the other components of the centrosomes are made.

Average Rating: 5.0 | 1 Scientist has reviewed this product

“ No contamination ”
Review: The cells from Creative Bioarray were free from contamination, which help me obtain reliable experimental outcomes.
Review date: 16 June 2023.

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DOHH-2

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