Calu-3

Specification
Recommended Products

Cat.No.

CSC-C9348L

Description

Established from a 25-year-old Caucasian male. The patient had received prior therapy with cytoxan, bleomycin and adriamycin. This cell line is tumorigenic and forms well differentiated grade I adenocarcinoma in nude mice.

Species

Homo sapiens (human)

Tissue of Origin

Lung

Recommended Medium

EMEM

Culture Properties

Adherent

Morphology

Epithelial

STR DNA Profile

Amelogenin: X
CSF1PO: 11,12
D13S317: 12
D16S539: 12,14
D5S818: 11
D7S820: 10,11
THO1: 6,9.3
TPOX: 8
vWA: 16,17

Karyotype

The stemline chromosome number is hypotriploid with the 2S component occurring at 1.4%. Approximately 20 markers were common to most S metaphases, of which i (1p), t(12;?) and t(18;?) were generally paired; t(6t?) had an HSR segment of q arm, and M7 had two secondary constrictions. Normal chromosomes 1, 13, 15 and 17 were absent, and the X was disomic. No Y chromosome was detected in the QM stained preparations.

Applications

This cell line can be used as a suitable transfection host.

Disease

Lung adenocarcinoma

Quality Control

Tests for mycoplasma, bacteria and fungi were negative

Storage and Shipping

Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule
Shipping Condition: Room Temperature

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

CM-1041X

SuperCult® EMEM

INQUIRY

For research use only. Not for any other purpose.

Q & A
Customer Review

What is the MTT assay for cell viability?

The MTT assay is used to determine the cellular viability or metabolic activity in microcapsules. It is based on the ability of metabolically active cells to transform a water-soluble dye[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] into an insoluble formazan.

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Average Rating: 4.0 | 1 Scientist has reviewed this product

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Creative Bioarray provided prompt customer support and ensured a smooth delivery process.

23 May 2023

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