Established from a 25-year-old Caucasian male. The patient had received prior therapy with cytoxan, bleomycin and adriamycin. This cell line is tumorigenic and forms well differentiated grade I adenocarcinoma in nude mice.
Homo sapiens, human
STR DNA Profile
The stemline chromosome number is hypotriploid with the 2S component occurring at 1.4%. Approximately 20 markers were common to most S metaphases, of which i (1p), t(12;?) and t(18;?) were generally paired; t(6t?) had an HSR segment of q arm, and M7 had two secondary constrictions. Normal chromosomes 1, 13, 15 and 17 were absent, and the X was disomic. No Y chromosome was detected in the QM stained preparations.
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule
Shipping Condition: Room Temperature
This cell line can be used as a suitable transfection host.
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