Macrophage Medium (MaM) is a complete medium designed for optimal growth of normal bone marrow-derived macrophages in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes growth of normal macrophages in vitro.
Storage and Shipping
Store the basal medium at 4 °C, the MaGS, the FBS and the P/S solution at -20 °C. Protect from light. Gel ice.
MaM consists of 425 ml of basal medium, 25 ml of fetal bovine serum, 50 ml of macrophage growth supplement and 5 ml of penicillin/streptomycin solution.
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