Epithelial Cell Medium-animal (EpiCM-a) is a complete medium designed for optimal growth of normal animal epithelial cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (2%). The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes proliferation and growth of normal human epithelial cells in vitro.
Storage and Shipping
Store the basal medium at 4 °C, the EpiCGS-a, the FBS and the P/S solution at -20 °C. Protect from light. Gel ice.
EpiCM-a consists of 500 ml of basal medium, 10 ml of fetal bovine serum, 5 ml of epithelial cell growth supplement-animal, and 5 ml of penicillin/streptomycin solution.
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