ActoFactor™ Recombinant Rat Vascular endothelial growth factor 188

CSC-CTK0845

Mouse Vascular Endothelial Growth Factor 144 (VEGF-144), a 16.9 kDa protein consisting of 144 amino acid residues, is produced as a homodimer. VEGF-144 is a polypeptide growth factor and a member of the platelet-derived growth factor family. It is a specific mitogen for vascular endothelial cells and a strong angiogenic factor in vivo. Two high-affinity tyrosine kinase receptors for VEGF-144 have been identified, VEGFR-1 (FLT-1), and VEGFR-2 (Flk-1). Consistent with the endothelial cell-specific action of VEGF-144, expression of both receptor genes has been found predominantly but not exclusively on endothelial cells. Expression of VEGFR-1 was also found on human monocytes, neutrophils (PMNs), bovine brain pericytes and villous and extravillous trophoblasts. In addition to its action as a mitogen it is a potent vascular permeability factor (VPF) in vivo and is also a chemo attractant for monocytes and endothelial cells. At least four different proteins are generated by differential splicing of the mouse VEGF gene: VEGF-120, VEGF-144, VEGF-164 and VEGF-188. The most abundant form is VEGF-164. Whereas VEGF-120, VEGF-144 and VEGF-164 are secreted proteins, VEGF-188 is strongly cell-associated. In addition, the isoforms VEGF-164 and VEGF-188 bind to heparin with high affinity. VEGF is apparently a homodimer, but preparations of VEGF show some heterogeneity on SDS gels depending of the secretion of different forms and the varying degrees of glycosylation. All dimeric forms possess similar biological activities. A related protein of VEGF is placenta growth factor (PlGF) with about 53% homology and VEGF-B with similar biological activities. The recombinant mouse VEGF-188 is a 44.2 kDa protein containing 188 amino acid residues.

Rat

Rat Vegfa expressed in E.coli

44.2 kDa

CAT# CSC-CTK0845-5 (5 μg); CAT# CSC-CTK0845-25 (25 μg)

E.coli

Greater than 95% as determined by SDS-PAGE analysis.

The ED50 for stimulation of cell proliferation by human umbilical vein endothelial cells for VEGF188 has been determined to be in the range of 2-10 ng/ml.

Less than 1 EU/μg.

Lyophilized from a sterile-filtered protein solution in 50 mM acetic acid.

Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile 50 mM acetic acid to a concentration not lower than 50 μg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

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