ActoFactor™ Recombinant Rat Interleukin 7
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Cat.No.
CSC-CTK0821
Description
IL-7 is a hematopoietic growth factor which affects primarily early B and T cells. Produced by thymic stromal cells, spleen cells and keratinocytes, IL-7 can also co-stimulate the proliferation of mature T cells in combination with other factors such as ConA and IL-2. Human and murine IL-7 is cross-species reactive. Recombinant rat IL-7 is a 15.0 kDa protein containing 130 amino acid residues.
Species
Rat
Product Overview
Rat Il7 expressed in E.coli
Molecular Mass
15 kDa
Size
CAT# CSC-CTK0821-10 (10 μg); CAT# CSC-CTK0821-50 (50 μg)
Expression System
E.coli
Purity
Greater than 98% as determined by SDS-PAGE and HPLC analysis.
Activity
The ED50 was determined by the dose-dependent stimulation of the proliferation of murine 2E8 cells is 5 x 10^6 units/mg.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Sterile-filtered and lyophilized from 10 mM sodium acetate (pH 5.8) with no additives.
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rRa IL-7 should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Citation Guidance
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