ActoFactor™ Recombinant Mouse Fibroblast growth factor 15
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Cat.No.
CSC-CTK0667
Description
FGF-15 takes part in the suppression of bile acid biosynthesis by down-regulation of CYP7A1 expression. FGF-15 is expressed in the developing brain. FGF-15 produced in E. coli is a 23.78 kDa protein containing 203 amino acid residues including a 10 a.a. N-Terminal His-tag.
Species
                                Mouse
                            Product Overview
                                Mouse Fgf15 expressed in E.coli
                            Molecular Mass
                                23.8 kDa
                            Size
                                CAT# CSC-CTK0667-10 (10 μg); CAT# CSC-CTK0667-50 (50 μg)
                            Expression System
                                E.coli
                            Purity
                                Greater than 90% as determined by SDS-PAGE analysis.
                            Activity
                                N/A
                            Endotoxin Level
                                Less than 1 EU/μg.
                            Formulation
                                Lyophilized from a filtered (0.4 @m) protein soluti on (0.5 mg/ml) in 20 mM TRIS (pH 8.0) and 20 mM NaCl.
                            Reconstitution
                                Please centrifuge product before opening vial. It is recommended to add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it on cell culture. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
                            Storage & Stability
                                The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
                            Citation Guidance
                        
                            If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
                            
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