ActoFactor™ Recombinant Human Lectin, galactoside-binding, soluble, 8

CSC-CTK0584

Galectins are a family of animal lectins with an affinity for beta-galactosides. This family has at least 14 identified members. Galectins share similarities in the CRD (the carbohydrate recognition domain). Galectins are synthesized as cytosolic proteins. Though localized principally in the cytoplasm and lacking a classical signal peptide, galectins can also be stimulated to secretion by non-classical pathways or alternatively targeted to the nucleus. Galectins are involved in modulating cell-cell and cell-matrix interactions. Galectin-8 is a tandem-repeat-type member of the galectin family, consisting of 2 CRDs attached by a linker peptide. Galectin-8 is greatly expressed in lung carcinomas, a number of forms of prostate carcinomas, in addition to other tumor cells. Galectin-8 attaches to a subset of cell surface integrins to modulate ECM-integrin interactions. Once immobilized, Galectin-8 promotes cell adhesion by ligation and clustering of cell surface integrin receptors. On the other hand, as a soluble ligand, Galectin-8 can inhibit cell adhesion. Human recombinant Galectin-8 produced in E. coli is a single, non-glycosylated polypeptide chain of 317 amino acids and a molecular mass of 35.8 kDa. It has been purified by proprietary chromatographic techniques.

Human

Human LGALS8 expressed in E.coli

35.8 kDa

CAT# CSC-CTK0584-10 (10 μg); CAT# CSC-CTK0584-50 (50 μg)

E.coli

Greater than 95% as determined by SDS-PAGE and RP-HPLC analysis.

rHu Galectin-8 is fully biologically active when compared to a standard. The ED50 of Galectin-8 as determined by its ability to agglutinate human red blood cells is 0.8-4 μg/ml.

Less than 1 EU/μg.

Sterile-filtered, white, lyophilized (freeze-dried) powder. Lyophilized from a concentrated (1 mg/ml) protein solution in 20 mM PBS (pH 7.4).

Please centrifuge product before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

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