Human Interleukin-4 (rHu IL-4) is a 15 kDa protein containing 130 amino acid residues. rHu IL-4 is a potent lymphoid cell growth factor, which stimulates the growth and survivability of certain B-cells and T-Cells.|Human recombinant Interleukin-4 produced in CHO cells is a single, non- glycosylated polypeptide chain of 129 amino acids having a molecular mass of 14 kDa. The glycosylated IL-4 migrates as a 18 kDa band in SDS-PAGE analysis. The protein has been purified by using proprietary chromatographic techniques.
Human IL4 expressed in CHO cells
CAT# CSC-CTK0226-10 (10 μg); CAT# CSC-CTK0226-50 (50 μg)
Greater than 97% as determined by SDS-PAGE and HPLC analysis.
Measured in a cell proliferation assay using TF-1 cells. The ED50 for this effect is 0.05-0.2 ng/ml.
Lyophilized from sterile-filtered protein solution in phosphate-buffered saline (pH 7.4).
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted with distilled, sterile water to a concentration of 0.1-1.0 mg/ml. For further dilution, carrier protein (0.1% BSA or HSA) should be added to avoid loss of bioactivity.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu IL-4 should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze/thaw cycles.
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