At least 23 different variants of IFN-alpha are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end. Recombinant human Interferon-alpha (rHu IFN-alpha) is a lymphoid factor, which possesses potent anti-viral activity. rHu IFN-alpha 2b produced in HEK cells is a glycosylated monomer, having a total molecular weight of 16 kDa. It has been purified by proprietary chromatographic techniques.
Human IFNA2 expressed in HEK cells
CAT# CSC-CTK0183-10 (10 μg); CAT# CSC-CTK0183-50 (50 μg)
Greater than 95% as determined by SDS-PAGE analysis.
rHu IFN-alpha 2b is fully biologically active when compared to a standard. The specific activity was determined by the dose-dependent cytotoxicity of the human TF-1 cell line (human erythroleukemic indicator cell line) and is typically 0.02-0.08 ng/ml.
Less than 1 EU/μg.
Sterile-filtered, white, lyophilized (freeze-dried) powder. Lyophilized from a concentrated protein solution (1 mg/ml) in 1xPBS.
Please centrifuge product briefly before opening the vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted IFN-alpha 2b should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze / thaw cycles.
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