gAcrp30 is a naturally occurring globular protein, obtained by proteolytic processing of adiponectin. Adiponectin is produced and secreted exclusively by adipocytes, and is a relatively abundant plasma protein, accounting for up to 0.05% of total serum protein. Like Adiponectin, gAcrp30 is capable of decreasing hyperglycemia and reversing insulin resistance. Additionally, gAcrp30 has been shown to be an important factor in promoting fat loss by signaling muscle to absorb and burn Free-Fatty Acids (FFAs). The signaling receptors for adiponectin and gAcrp30 have recently been identified and names AdipoR1 and AdipoR2. AdipoR2 is predominantly expressed in the liver. Recombinant human gAcrp30/Adipolean is a 16.6 kDa protein consisting of 145 amino acid residues.
Human ADIPOQ expressed in E.coli
CAT# CSC-CTK0502-25 (25 μg); CAT# CSC-CTK0502-125 (125 μg)
Greater than 98% as determined by SDS-PAGE and HPLC analysis.
Determined by its ability to inhibit the proliferation of murine myeloid cell lines M1. The ED50 for this effect is 1 - 2.5 μg/ml.
Less than 2EU/μg.
Lyophilized from a sterile-filtered solution (1 mg/ml) in 10 mM Tris (pH8) and 0.75 mM DTT.
Please centrifuge product briefly before opening the vial. Reconstitute in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted gAcrp30 should be used immediately or stored in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.
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