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ActoFactor™ Recombinant Human Collagen, type XVIII, alpha 1

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Cat.No.
CSC-CTK0614
Description
Endostatin is a naturally occurring 20 kDa polypeptide derived from the C-terminal portion of type XVIII collagen. It functions as an anti-angiogenic cytokine that is expressed in various organs with the highest levels in liver, lung and kidney. Endostatin inhibits angiogenesis by blocking the pro-angiogenic activities of VEGF and FGF-basic. Recombinant human Endostatin produced in Pichia Pastoris is a single, glycosylated polypeptide consisting of 184 amino acid residues and having a total molecular mass of 20,000 Dalton. is a 20.2 kDa protein. The Endostatin was purified using proprietary chromatographic techniques.
Organism
Human
Product Overview
Human COL18A1 expressed in Pichia pastoris
Molecular Mass
20.2 kDa
Size
CAT# CSC-CTK0614-100 (100 μg); CAT# CSC-CTK0614-500 (500 μg)
Expression System
Pichia pastoris
Purity
Greater than 98% as determined by SDS-PAGE and HPLC analysis.
Activity
The activity calculated by ECE migration inhibition was found to be 50,000IU/mg.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Sterile filtered liquid formulation 5 mg/ml in PBS.
Reconstitution
N/A
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored dessicated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at –20°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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