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Immortalized Mouse Renal Proximal Tubule Cells
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Cat.No.
CSC-I9243L
Description
Immortalized Mouse Renal Proximal Tubule Cells-SV40 have been obtained immortalizing Mouse Renal Proximal Tubule Cells with Lenti-SV40 Lentivirus. Immortalized cells were controlled passaging side by side with the primary cells. Primary cells go into senescence after the 4th passage while the SV40 tranduced cells go beyond 20 passges.
Species
Mus musculus
Source
Kidney
Culture Properties
Adherent
Morphology
Cobblestone
Immortalization Method
Isolated from mouse carrying a simian virus 40 tumor antigen
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CSC-C9369W Mouse Renal Proximal Tubule Cells
CIK-HT003 HT? Lenti-SV40T Immortalization Kit
CIK-HT003 HT? Lenti-SV40T Immortalization Kit
Quality Control
1) Passaging for >20 passages before deeming them immortal.
2) qPCR analysis on SV40T expression.
3) Morphology assessment.
4) Negative for microbial contaminants.
2) qPCR analysis on SV40T expression.
3) Morphology assessment.
4) Negative for microbial contaminants.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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