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Immortalized Mouse Prostate Epithelial Cells (iMPEC)
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Cat.No.
CSC-I9181L
Description
The Immortalized Mouse Prostate Epithelial Cells (iMPEC, clone7) is derived by co-expression of adenovirus E1A and dominant-negative p53 (p53DD). In addition to its non-tumorigenicity, this cell line maintains its primary phenotype and expresses tissue-specific markers such as the androgen receptor and the homeobox protein Nkx3.1. This cell line is a useful model for studying prostate growth regulation.
Species
Mus musculus
Source
Prostate glands
Culture Properties
Adherent
Morphology
Cuboidal
Immortalization Method
Serial passaging and electroporation transfection with linearized DNA of E1A and p53DD
Markers
Androgen receptor, Nkx3.1, β-catenin, CK8, CK18 and vimentin
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
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CSC-C9074J BALB/c Mouse Prostate Epithelial Cells
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
1. Western blot was used to confirm the presence of E1A and p53DD gene in the immortalized cell line.
2. Western blot was used to check tissue-specific markers.
2. Western blot was used to check tissue-specific markers.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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