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Immortalized Mouse Kidney Epithelial Cells (iBMK)
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Cat.No.
CSC-I9186L
Description
The Immortalized Baby Mouse Kidney Epithelial Cells (iBMK, Clone W2) is derived by co-expression of adenovirus E1A and dominant-negative p53 (p53DD). In addition to its non-tumorigenicity, this cell line maintains its primary phenotype and expresses epithelial-specific markers such as E-cadherin, cytokeratin 8 and 18. Together with Bax Knockdown iBMK, Bak Knockdown iBMK and Bax/Bak Double Knockdown iBMK, these cell lines form the ideal system for studying mechanisms regulating apoptosis. In the same way, when paired with Atg5 Knockdown iBMK and Atg7 Knockdown iBMK, they present an attractive model for investigating the relationship between autophagy and metabolism homeostasis.
Species
Mus musculus
Source
Kidney
Culture Properties
Adherent
Morphology
Cuboidal
Immortalization Method
Serial passaging and electroporation transfection with linearized DNA of E1A and p53DD
Markers
E-cadherin, CK8 and CK18
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
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CSC-C9050J B129 Mouse Kidney Epithelial Cells
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CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
1. Western blot was used to confirm the presence of E1A and p53DD gene in the immortalized cell line.
2. Western blot was used to check epithelial markers E-cadherin and cytokeratin expression.
2. Western blot was used to check epithelial markers E-cadherin and cytokeratin expression.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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