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Immortalized Mouse CD4
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Cat.No.
CSC-I9242L
Description
The Immortalized Mouse CD4+CD8+T Cells (MOHITO) has characteristics of an immortalized cell line, but are not immortalized by any immortalization reagents. It expresses the Notch1 and Jak1 mutations, as well as TCR rearrangement, both of which are characteristics to human T-cell acute lymphoblastic leukemia (T-ALL). The cells are derived from CD4+CD8+T cells and are interleukin-7 (IL-7) dependent. The presence of IL-7 and IL-2 are required to activate the JAK/STAT signaling pathway. Transfection with BCR-ABL1 or mutant JAK1 transforms the cells to become IL-7 independent for proliferation. These cells are able to induce T-ALL-like disease when injected into healthy Balb/c mice. This cell line represents a novel model system to study T cell-specific protein signaling and inhibition mechanisms and oncogenic mutations, moreover they can be utilized inin vitroandin vivopharmaceutical studies.
Species
Mus musculus
Source
Spleen
Culture Properties
Suspension
Morphology
Round
Immortalization Method
Spontaneous immortalization
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Quality Control
1) Flow cytometry to analyze CD4+ CD8+ phenotype;
2) PCR assay for T cell receptor expression;
3) Sequence analysis to identify point mutations in Notch1 and Jak1
2) PCR assay for T cell receptor expression;
3) Sequence analysis to identify point mutations in Notch1 and Jak1
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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