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Immortalized (Conditionally) Mouse Lung Clara Cells (C22)
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Cat.No.
CSC-I9178L
Description
Clara cells play a role in the maintenance of normal bronchiolar epithelium by secreting Clara Cell Secretory Proteins (i.e. CC10) to protect the respiratory tract against oxidative stress and inflammation. The C22 is a conditionally immortalized mouse lung clara cells isolated from the mouse harboring thermolabile mutation (tsA58) under the control of an interferon (IFN)-γ-inducible H-2Kb promoter and a temperature-sensitive simian virus 40 large T antigen. The functional expression of the SV40 large T antigen is induced by culturing the cellsin vitroin medium containing IFN γ at a temperature permissive (33°C). At a non-permissive temperature (37°C-39°C), the cells cease to proliferate.C22 resembles closely to primary clara cells and constitutively expresses and secretes surfactant proteins SP-A, SP-B, SP-D and CC10. This cell line is useful in studying lung epithelial physiology, as well as studying the role of CC10 in pulmonary inflammation and viral infection.
Species
Mus musculus
Source
Lung
Culture Properties
Adherent
Morphology
Under proliferation phase: cell size varies from 10 to 25 μm. Smaller cells are spindle shaped with clear cytoplasma and grow at a slower rate than larger cells, which are rounded in shape with a vacuolated cytoplasma and a faster doubling time.Upon differentiation: cells increase in diameter and acquire clear to foamy vacuoles.
Immortalization Method
Isolated from transgenic mouse carrying a temperature-sensitive simian virus 40 tumor antigen (tsA58)
Markers
1) Presence of CC10, SP-A, SP-B and absence of SP-C confirmed by RT-PCR;
2) Synthesis and secretion of CC10, SP-A, SP-B and SP-D proteins confirmed by immunoblotting
2) Synthesis and secretion of CC10, SP-A, SP-B and SP-D proteins confirmed by immunoblotting
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
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BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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