The clonal cell line, RN46A-B14, was isolated following transfection of the gene encoding rat brain-derived neurotrophic factor (BDNF) into RN46A cells. RN46A-B14 cells synthesize and secrete biologically active BDNF in vitro and synthesize serotonin (5-HT) following partial membrane depolarization. Two weeks following RN46A-B14 cell transplantation into the adult rat cortex and hippocampus, there is a threefold increase in survival of RN46A-B14 cells compared to RN46A cells. The grafted RN46A-B14 cells immunohistochemically stain for BDNF and 5-HT, while RN46A cells transfected with vector only are negative for both BDNF and 5HT. In addition, RN46A-B14 cells attain more morphologically complex phenotypes, indicating enhanced neuronal differentiation. Autocrine secretion of BDNF by RN46A-B14 cells thus potentiates survival and can be used to deliver both BDNF and 5-HT in vivo.
Tissue of Origin
Embryonic rat (day 13) medullary raphe
DMEM: Ham's F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.25mg/ml Geneticin (G418) + 0.1mg/ml hygromycin. Alternatively CNS medium can be used .
Liquid Nitrogen (-180 °C).
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Never can cryopreserved cells be kept at -20 °C.
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