Established from the alveololar rhabdomyosarcoma of the liver of a girl after chemotherapy; cells were described to contain a deletion mutation of p53 and to be resistant to FAS- and TRAIL-induced apoptosis
adherent, epithelial-like cells growing as monolayers
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, cytokeratin-19 -, desmin +, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin +
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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