Established from the long-term bone marrow cultures of BDF1 mice; cells are mIL-3-dependent (FDCP means "factor dependent cell-Paterson" after the Paterson Institute, Manchester, UK); it is described that cells can be maintained in a primitive state or induced to differentiate
Murine near-diploid karyotype with 10% polyploidy - 40(38-40)<2n> - fusion markers absent
round, single cells in suspension, sometimes in small clusters
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 3-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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