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FDCP-Mix cl.A4
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Cat.No.
CSC-C2762
Description
Established from the long-term bone marrow cultures of BDF1 mice; cells are mIL-3-dependent (FDCP means "factor dependent cell-Paterson" after the Paterson Institute, Manchester, UK); it is described that cells can be maintained in a primitive state or induced to differentiate
Species
Mouse
Source
bone marrow
Recommended Medium
80% SuperCult® IMDM + 20%horse serum + 10 ng/ml murine IL-3 (or 10-20% conditioned medium of cell line WEHI-3B)
Culture Properties
suspension
Morphology
round, single cells in suspension, sometimes in small clusters
Karyotype
Murine near-diploid karyotype with 10% polyploidy - 40(38-40)<2n> - fusion markers absent
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 3-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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