Recently proposed as a potential model of atherosclerosis, allograft rejection and accommodation, xenotransplantation and viral haemorrhage diseases, the porcine endothelial cells provide a useful tool in studies including the molecular mechanisms of xenogeneic rejection in the swine-to-human combination, and the pathogenesis of African Swine Fever (ASF) and Classical Swine Fever (CSF). The Immortalized Porcine Aortic Endothelial Cells (AOC) is derived from stable transformation of SV40 genome into primary porcine aortic endothelial cells. The resulting AOC retains comparable surface markers to its primary cell counterparts, as well as preserving its ability to uptake acetylated low density lipoproteins (Ac-LDL) and its susceptibility to xenogeneic cell-mediated cytotoxicity (i.e. Human Natural Killer Cell-mediated lysis).
Mus musculus, Large White Swine
Tranfection with pRNS-1 plasmid encoding the SV40 genome
CD29, CD31, CD41/61, CD80/86, CD46, SWC3, LAMP-1
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
CSC-C1755 Porcine Aortic Endothelial Cells
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
For Research Use Only
1) Flow cytometry was used to confirm the expression of endothelial cell markers such as CD29, CD31, CD41/61, CD80/86, CD46, SWC3, LAMP-1 antigen and MHC Class I antigens;
2) 51Cr release assay was used to analyse the susceptibility of AOC to xenogeneic cell-mediated cytotoxicity;
3) 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine (DiI) -labelled Ac-LDL was used to evaluate Ac-LDL update by the AOC.
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