Description
Recently proposed as a potential model of atherosclerosis, allograft rejection and accommodation, xenotransplantation and viral haemorrhage diseases, the porcine endothelial cells provide a useful tool in studies including the molecular mechanisms of xenogeneic rejection in the swine-to-human combination, and the pathogenesis of African Swine Fever (ASF) and Classical Swine Fever (CSF). The Immortalized Porcine Aortic Endothelial Cells (AOC) is derived from stable transformation of SV40 genome into primary porcine aortic endothelial cells. The resulting AOC retains comparable surface markers to its primary cell counterparts, as well as preserving its ability to uptake acetylated low density lipoproteins (Ac-LDL) and its susceptibility to xenogeneic cell-mediated cytotoxicity (i.e. Human Natural Killer Cell-mediated lysis).
Organism
Mus musculus, Large White Swine
Culture Properties
Adherent
Morphology
Cobblestone-like
Immortalization Method
Tranfection with pRNS-1 plasmid encoding the SV40 genome
Markers
CD29, CD31, CD41/61, CD80/86, CD46, SWC3, LAMP-1
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Recommended Products
CSC-C1755 Porcine Aortic Endothelial Cells
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Applications
For Research Use Only
Quality Control
1) Flow cytometry was used to confirm the expression of endothelial cell markers such as CD29, CD31, CD41/61, CD80/86, CD46, SWC3, LAMP-1 antigen and MHC Class I antigens;
2) 51Cr release assay was used to analyse the susceptibility of AOC to xenogeneic cell-mediated cytotoxicity;
3) 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine (DiI) -labelled Ac-LDL was used to evaluate Ac-LDL update by the AOC.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
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