Immortalized Porcine Aortic Endothelial Cells (AOC)

Cat.No.: CSC-I9192L

Species: Mus musculus

Source: Aorta

Morphology: Cobblestone-like

Culture Properties: Adherent

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Scientific Data
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Cat.No.

CSC-I9192L

Description

Recently proposed as a potential model of atherosclerosis, allograft rejection and accommodation, xenotransplantation and viral haemorrhage diseases, the porcine endothelial cells provide a useful tool in studies including the molecular mechanisms of xenogeneic rejection in the swine-to-human combination, and the pathogenesis of African Swine Fever (ASF) and Classical Swine Fever (CSF). The Immortalized Porcine Aortic Endothelial Cells (AOC) is derived from stable transformation of SV40 genome into primary porcine aortic endothelial cells. The resulting AOC retains comparable surface markers to its primary cell counterparts, as well as preserving its ability to uptake acetylated low density lipoproteins (Ac-LDL) and its susceptibility to xenogeneic cell-mediated cytotoxicity (i.e. Human Natural Killer Cell-mediated lysis).

Species

Mus musculus

Source

Aorta

Culture Properties

Adherent

Morphology

Cobblestone-like

Immortalization Method

Tranfection with pRNS-1 plasmid encoding the SV40 genome

Markers

CD29, CD31, CD41/61, CD80/86, CD46, SWC3, LAMP-1

Application

For Research Use Only

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Note: Never can cells be kept at -20 °C.

Shipping

Dry Ice.

Recommended Products

CSC-C1755 Porcine Aortic Endothelial Cells
CIK-HT003 HT® Lenti-SV40T Immortalization Kit

Quality Control

1) Flow cytometry was used to confirm the expression of endothelial cell markers such as CD29, CD31, CD41/61, CD80/86, CD46, SWC3, LAMP-1 antigen and MHC Class I antigens;
2) 51Cr release assay was used to analyse the susceptibility of AOC to xenogeneic cell-mediated cytotoxicity;
3) 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine (DiI) -labelled Ac-LDL was used to evaluate Ac-LDL update by the AOC.

BioSafety Level

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Immortalized Porcine Aortic Endothelial Cells (AOC) are a spontaneously immortalized cell line obtained from aortic endothelium of a Large White pig. Primary endothelial cells have a limited ability for proliferation, however this cell line was generated via serial passage over a lengthy period until continuous proliferation was achieved, giving it a stable and scalable model for vascular research.

In vitro, the AOC cells maintain the morphological and functional features of artery endothelial cells and create a homogeneous monolayer of polygonal cells. They express major endothelium markers including von Willebrand Factor (vWF), CD31 and VE-Cadherin and retain physiological capabilities including thrombin-induced prostacyclin production. They are cultured in a regular basal medium with added serum under conventional conditions (37°C, 5% CO₂).

Because of their porcine origin and human-like cardiovascular physiology, AOC cells are frequently employed in xenotransplantation research, especially in the study of endothelial activation and molecular causes of acute vascular rejection. They also provide a powerful platform to study atherosclerosis, thrombosis, vascular inflammation and shear stress on endothelial biology.

Nanoparticle Interaction with Aortic Endothelial Cells Stimulates VEGF Secretion

Since several research underlined an increased cardiovascular risk due to NPs, present study was undertaken to investigate their effect on aortic endothelial cells (AOC).

Fluorescence microscopy revealed that NPs specifically colocalized with AOCs at all tested concentrations (5-75 µg/mL), with uptake increasing significantly at higher doses (25 and 75 µg/mL) compared to the lowest concentration (Fig. 1). While NP treatment did not alter cell proliferation (Fig. 2A), it significantly stimulated metabolic activity, as measured by ATP production (Fig. 2B). Similarly, VEGF secretion was significantly increased by all NP concentrations (Fig. 3); however, this effect occurred independently of changes in VEGFgene expression (Fig. 4). These findings suggest that NPs can directly interact with endothelial cells and modulate their secretory function.

The panel shows representative images of AOC treated with different concentrations of NPs (negative control C, 5, 25, 75 µg/mL). — Fig. 1. The panel shows representative images of AOC treated with different concentrations of NPs (negative control C, 5, 25, 75 µg/mL) (Basini G, Grolli S, *et al.*, 2023).

Effect of the treatment with nanoplastics (NPs 5, 25 and 75 µg/mL) for 48 h on swine aortic endothelial cells (AOC) proliferation using 5-bromo-2'-deoxyuridine (BrdU) incorporation assay test (A) and metabolic activity using ATP content assay test (B). — Fig. 2. Effect of the treatment with nanoplastics (NPs 5, 25 and 75 µg/mL) for 48 h on swine aortic endothelial cells (AOC) proliferation using 5-bromo-2'-deoxyuridine (BrdU) incorporation assay test (A) and metabolic activity using ATP content assay test (B) (Basini G, Grolli S, *et al.*, 2023).

Fig. 4. This figure shows the ΔCt values of gene expression of VEGF, SOD, iNOS, and eNOS in AOC treated with 75 µg/mL of NPs (NPs) compared to AOC cultured in standard conditions (Control) (Basini G, Grolli S, *et al.*, 2023).

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For research use only. Not for any other purpose.