Pathway Determination Assay
Metabolic evaluation in the early stage of drug development and lead optimization has become increasingly important. Learning how a compound is eliminated in the human body is quite useful to assess the potential of drug-drug interactions. Pathway determination assay provides critical information regarding the metabolized fraction of drug candidates, which help researchers understand the mechanisms of drug metabolism.
Our scientific team has decades of experience to offer whole in vitro metabolism assay packages for new compounds. We have state-of-the-art instrument and detailed lab services.
Pathway Determination Assay Protocol
| Test system | Human liver microsomes; recombinant human CYPs; recombinant human UGTs |
| Test Compound Concentration | 3 µM (or custom) |
| Time Points | 0, 5, 15, 30, 45, 60 minutes |
| Number of Replicates | 3 |
| Readout | Proposed metabolic pathway metabolite abundance |
| Compound Requirements | 500 µL of 10 mM solution |
| Analysis Method | LC-MS/MS or LC-UV |
| Data Delivery | Remaining of parent and amount of metabolite formation, Half life, Standard error of half life |
Creative Bioarray is dedicated to providing comprehensive services with high effectivity and reliability to accelerate drug development. If you have any special needs or questions regarding our services, please feel free to contact us. We look forward to cooperating with you in the future.
