ADME, an abbreviation for absorption, distribution, metabolism, and elimination, describes the disposition of drug candidates within an organism. ADME determine the drug levels and kinetics within a body, and hence influence the performance and bioactivity of the compound as a drug. Therefore, ADME assessment of candidate compounds is a crucial step in drug development before going to clinical trials.
Creative Bioarray offers standard, predictive and comprehensive cell-based ADME tests by utilization of the state-of-the-art techniques, industry leading products and equipment. We are dedicated to helping drug developers reduce cost and time-to-market by providing reliable and fast data. The service groups at Creative Bioarray are highly skilled in absorption, transport metabolism studies, which enable us to develop a wide range of ADME tests to meet the customers’ special needs.
Permeability assays are important to understand the absorption properties of your compounds for oral dosing. Intestinal epithelium permeability is a crucial to determine the rate and extent of compound absorption and thus influences its bioavailability. Creative Bioarray uses polarized cell monolayers formed by Caco-2 cell line to estimate the ability of candidate compounds to cross the intestinal epithelium, which represents for the golden standard model for permeability evaluation in pharmaceutical industries. Caco-2 cells, originated from a human colon epithelial cancer cell line, are characterized by mimicking small intestinal epithelium in morphology and functions, such as the ability to form a polarized monolayer, expression of phase II conjugation enzymes and transporters.
★ Testing procedure
Caco-2 cells are grown to a confluent monolayer on a supportive surface. The testing compound is added to the apical side (A) of the Caco-2 cell monolayer, and the test compound on the basolateral side (B) is monitored to determine permeability. Bi-directional assessment (A to B and B to A) is also available and then efflux ratio (ER) is calculated to indicate whether active efflux exists.