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Creative Bioarray is a reliable MDR1-MDCK permeability assay provider. After years of development, Creative Bioarray has become an expert in the field of drug discovery and development. By using a highly automated approach, we offer this in vitro ADME screening service to determine drug permeability.
Modern drug discovery involves the identification of screening from a mass of candidate compounds by using high-throughput technology, ADME screening (absorption, distribution, metabolism, and excretion), then once a compound that fulfills all of these requirements has been identified, it will begin the process of drug development prior to clinical trials.
An investigational drug should be evaluated in vitro to determine whether they are a potential substrate of P-glycoprotein (a well-recognized efflux transporter in many tissues and organs such as brain, kidney and intestine). MDR1-MDCK permeability assay is one of drug absorption screening methods. This method uses MDR1-MDCK cell line which is a combination of Madin Darby Canine Kidney (MDCK) cells and MDR1 gene to encode the efflux protein, P-gp. The MDR1-MDCK permeability assay is a valuable tool to identify and characterize P-gp substrates and inhibitors, it can help understand the mechanism of drug efflux and highlight early potential issues with drug permeability at an early stage.
Compared to Caco-2 cells, MDCK cells can form cell monolayer with tight junction much faster, with lower transporters ex
Fig. The schematic diagram of MDR1-MDCK model
The PAMPA experimental protocol is as follows:
|1)||MDCK (Madin-Darby Canine Kidney) cells are seeded on a Multiscreen plate to form a confluent monolayer for four days|
|2)||Monolayer integrity is checked with TEER (Transepithelial Electrical Resistance) measurement|
|3)||After washing with HBSS buffer, test compounds (10 μM) are added to the apical side of the monolayers, together with leakage indicator Lucifer Yellow|
|4)||Incubate at 37°C in cell incubator for 1 hour|
|5)||Leakage of monolayer (Lucifer Yellow) is determined by fluorescent reader|
|6)||Basolateral samples in the receiving plate are transferred to a fresh plate for LC/MS/MS analysis|
Creative Bioarray offers consistent and cost effective MDR1-MDCK permeability assay service. With multiple specialists and years of experience in this area, we guarantee you quality data and customized results to meet your project requirements. We also provide stable MDR1-MDCK cell lines, you can just order this product. If you have additional requirements or questions, do not hesitate to contact us. Our specialists in this area will help you solve your problems, and Creative Bioarray will be your best partner in the field.